The TMC5 Antibody (CAB16144) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the TMC5 protein, researchers can accurately detect and analyze its expression in different cell types, making it ideal for investigations in molecular biology and cell biology.TMC5 is known for its role in ion transport and calcium signaling within cells, making it a potential target for understanding diseases related to these pathways, such as neurological disorders and certain cancers.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TMC5 Antibody
SKU:
CAB16144
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
YWQI TEGR KIMI RLLH EQII NEGK DKMF LIEK LIKL QDME KKAN PSSL VLER REVE QQGF LHLG EHDG SLDL RSRR SVQE GNPR A
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TMC5
Positive Sample:
BT-474
Cellular Localization:
Membrane, Multi-Pass Membrane Protein.
Calculated MW:
115kDa
Observed MW:
114kDa
Predicted to enable mechanosensitive ion channel activity. Predicted to be involved in ion transmembrane transport. Located in extracellular exosome.
Purification Method
Affinity purification
Gene ID
79838
RRID
AB_2763591
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from BT-474 cells, using TMC5 Rabbit pAb (CAB16144) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
