The TMED2 Antibody (CAB17622) is a high-quality antibody developed for reliable detection and analysis of target proteins. Involved in several processes, including Golgi organization; negative regulation of GTPase activity; and protein localization to plasma membrane. Located in Golgi apparatus; endoplasmic reticulum; and endoplasmic reticulum-Golgi intermediate compartment.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TMED2 Antibody
SKU:
CAB17622
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p24, P24A, RNP24, p24b1, p24beta1, TMED2
Positive Sample:
U-87MG, HepG2, Mouse liver, Mouse kidney, Rat spleen, Rat lung, Rat liver
Cellular Localization:
Copi-Coated Vesicle, Copii-Coated Er To Golgi Transport Vesicle, Endoplasmic Reticulum, Endoplasmic Reticulum-Golgi Intermediate Compartment, Golgi Apparatus, Transport Vesicle, Zymogen Granule Membrane.
Calculated MW:
23kDa
Observed MW:
23kDa
Involved in several processes, including Golgi organization; negative regulation of GTPase activity; and protein localization to plasma membrane. Located in Golgi apparatus; endoplasmic reticulum; and endoplasmic reticulum-Golgi intermediate compartment.
Purification Method
Affinity purification
Gene ID
10959
RRID
AB_2772632
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TMED2 Rabbit pAb (CAB17622) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunofluorescence analysis of C6 cells using TMED2 Rabbit pAb (CAB17622) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
