The TMEM147 Antibody (CAB17076) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables ribosome binding activity. Located in endoplasmic reticulum membrane. Part of protein-containing complex.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
TMEM147 Antibody
SKU:
CAB17076
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NEDFLPH, NIFIE14, TMEM147
Positive Sample:
MCF7
Cellular Localization:
Plasma Membrane.
Calculated MW:
25kDa
Observed MW:
25kDa
Enables ribosome binding activity. Located in endoplasmic reticulum membrane. Part of protein-containing complex.
Purification Method
Affinity purification
Gene ID
10430
RRID
AB_2772637
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from MCF7 cells, using TMEM147 Rabbit pAb (CAB17076) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
