TMEM14A Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
TMEM14A Antibody (PACO30486)
SKU:
PACO30486
Size:
50μg
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant Human Transmembrane protein 14A protein (45-78AA)
Immunogen Species:
Homo sapiens (Human)
Uniprot No:
Q9Y6G1
Form:
Liquid
Tested Applications:
ELISAWBIHCIF
Recommended Dilution:
WB 1:500-1:5000, IHC 1:200-1:500, IF 1:50-1:200
Synonyms:
TMEM14A, C6orf73, PTD011, Transmembrane protein 14A
IHC image of PACO30486 diluted at 1:350 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO30486 diluted at 1:350 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot All lanes: TMEM14A antibody at 0.8µg/ml + PC-3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 11 kDa Observed band size: 11 kDa
Immunofluorescent analysis of PC-3 cells using PACO30486 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
