The TNFRSF11A Antibody (CAB12997) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high specificity and reactivity with human samples, making it ideal for use in Western blot applications. By binding to the TNFRSF11A protein, this antibody enables accurate detection and analysis in various cell types, supporting research in areas such as immunology and bone biology.TNFRSF11A, also known as RANK, plays a crucial role in osteoclast differentiation and activation, making it a key target in studies related to bone health and diseases such as osteoporosis and bone cancer.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TNFRSF11A Antibody
SKU:
CAB12997
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
66kDa
Observed MW:
80kDa
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus.
Purification Method
Affinity purification
Gene ID
8792
RRID
AB_2759845
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TNFRSF11A Rabbit pAb (CAB12997) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of L929 cells using TNFRSF11A Rabbit pAb (CAB12997) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
