The TNIP1 Polyclonal Antibody (PAC020729) is specifically designed for research involving TNIP1, a key protein involved in the regulation of inflammation and immune responses. This antibody, generated in rabbits, is highly sensitive and specific for detecting TNIP1 in human samples, making it ideal for use in Western blot applications.TNIP1, also known as TNFAIP3 interacting protein 1, is a crucial mediator of immune system regulation, playing a significant role in controlling inflammation and autoimmune responses. Dysregulation of TNIP1 has been implicated in various inflammatory diseases, making it a promising target for research in immunology and autoimmune disorders.
By binding to TNIP1, this antibody allows for precise detection and analysis of TNIP1 levels in different cell types, providing researchers with valuable insights into its function and potential therapeutic implications. Investigating the role of TNIP1 is essential for advancing our understanding of immune system regulation and developing targeted treatments for inflammatory conditions and autoimmune diseases.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20729(TNIP1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 8%SDS-PAGE,Lysate: 40 μgPrimary antibody: PACO20729(TNIP1 Antibody) at dilution 1/400 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 20 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO20729(TNIP1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
This gene encodes an A20-binding protein which plays a role in autoimmunity and tissue homeostasis through the regulation of nuclear factor kappa-B activation. Mutations in this gene have been associated with psoriatic arthritis, rheumatoid arthritis, and systemic lupus erythematosus. Multiple transcript variants encoding different isoforms have been found for this gene.
Synonyms:
TNFAIP3 interacting protein 1
UniProt Protein Function:
TNIP1: Interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal; however, binding to A20/TNFAIP3 seems not to be required for this function. Inhibits NF-kappa-B activation by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcricption. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflamation; this function is mediated by association with SELPLG and dependent on phosohorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection. Interacts with TNFAIP3 and IKBKG; facilitates TNFAIP3- mediated de-ubiquitination of NEMO/IKBKG. Interacts with HIV-1 matrix protein. Interacts with Shigella flexneri ipah9.8; the interaction promotes polyubiquitination of IKBKG. Interacts with polyubiquitin. Interacts with MAPK1, SELPLG and PIK3CD. Interacts with IKBKG (polyubiquitinated). Interacts with IRAK1 (polyubiquitinated). Interacts with MYD88; the interaction is indicative for participation in an activated TLR-signaling complex. Ubiquitous. Strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Transcription regulation
Chromosomal Location of Human Ortholog: 5q32-q33.1
Molecular Function:protein binding; ubiquitin-specific protease activity; mitogen-activated protein kinase binding
Biological Process: MyD88-dependent toll-like receptor signaling pathway; leukocyte adhesion; negative regulation of viral genome replication; glycoprotein biosynthetic process; translation; defense response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; positive regulation of inflammatory response
NCBI Summary:
This gene encodes an A20-binding protein which plays a role in autoimmunity and tissue homeostasis through the regulation of nuclear factor kappa-B activation. Mutations in this gene have been associated with psoriatic arthritis, rheumatoid arthritis, and systemic lupus erythematosus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
