Description
TNIP1 Antibody (PACO57128)
The TNIP1 Polyclonal Antibody (PAC057128) is a valuable tool for researchers studying TNIP1, a key regulator of immune and inflammatory responses. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting TNIP1 in human samples, making it ideal for use in Western blot applications. By binding to TNIP1, this antibody enables precise detection and analysis in various cell types, providing valuable insights for immunology and inflammatory disease research.TNIP1, also known as ABIN-1, plays a critical role in modulating immune signaling pathways and inflammation. Dysregulation of TNIP1 has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and cancer.
Understanding the function of TNIP1 is essential for developing targeted therapies that can effectively modulate immune responses in these disease settings.Overall, the TNIP1 Polyclonal Antibody is a valuable tool for researchers seeking to further understand the role of TNIP1 in immune regulation and disease pathogenesis. Its high specificity and sensitivity make it a reliable option for investigating TNIP1 expression and function in a variety of experimental contexts.
| Antibody Name: | TNIP1 Antibody (PACO57128) |
| Antibody SKU: | PACO57128 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human, Rat |
| Immunogen: | Recombinant Human TNFAIP3-interacting protein 1 protein (526-636AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Rat kidney tissue. All lanes: TNIP1 antibody at 7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 72, 67, 65, 63, 62 kDa. Observed band size: 72 kDa. |
 | Immunofluorescence staining of Hela cells with PACO57128 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO57128 diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. |
| Background: | Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation; this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection. |
| Synonyms: | TNFAIP3-interacting protein 1 (A20-binding inhibitor of NF-kappa-B activation 1) (ABIN-1) (HIV-1 Nef-interacting protein) (Nef-associated factor 1) (Naf1) (Nip40-1) (Virion-associated nuclear shuttling protein) (VAN) (hVAN), TNIP1, KIAA0113 NAF1 |
| UniProt Protein Function: | TNIP1: Interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal; however, binding to A20/TNFAIP3 seems not to be required for this function. Inhibits NF-kappa-B activation by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcricption. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflamation; this function is mediated by association with SELPLG and dependent on phosohorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection. Interacts with TNFAIP3 and IKBKG; facilitates TNFAIP3- mediated de-ubiquitination of NEMO/IKBKG. Interacts with HIV-1 matrix protein. Interacts with Shigella flexneri ipah9.8; the interaction promotes polyubiquitination of IKBKG. Interacts with polyubiquitin. Interacts with MAPK1, SELPLG and PIK3CD. Interacts with IKBKG (polyubiquitinated). Interacts with IRAK1 (polyubiquitinated). Interacts with MYD88; the interaction is indicative for participation in an activated TLR-signaling complex. Ubiquitous. Strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription regulation Chromosomal Location of Human Ortholog: 5q32-q33.1 Cellular Component: cytoplasm; intracellular; nucleus Molecular Function:protein binding; ubiquitin-specific protease activity; mitogen-activated protein kinase binding Biological Process: MyD88-dependent toll-like receptor signaling pathway; leukocyte adhesion; negative regulation of viral genome replication; glycoprotein biosynthetic process; translation; defense response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; positive regulation of inflammatory response |
| NCBI Summary: | This gene encodes an A20-binding protein which plays a role in autoimmunity and tissue homeostasis through the regulation of nuclear factor kappa-B activation. Mutations in this gene have been associated with psoriatic arthritis, rheumatoid arthritis, and systemic lupus erythematosus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | Q15025 |
| NCBI GenInfo Identifier: | 20138952 |
| NCBI Gene ID: | 10318 |
| NCBI Accession: | Q15025.2 |
| UniProt Related Accession: | Q15025 |
| Molecular Weight: | Observed: 72 kDaPredicted: 72 kDa |
| NCBI Full Name: | TNFAIP3-interacting protein 1 |
| NCBI Synonym Full Names: | TNFAIP3 interacting protein 1 |
| NCBI Official Symbol: | TNIP1Â Â |
| NCBI Official Synonym Symbols: | VAN; NAF1; ABIN-1; nip40-1Â Â |
| NCBI Protein Information: | TNFAIP3-interacting protein 1 |
| UniProt Protein Name: | TNFAIP3-interacting protein 1 |
| UniProt Synonym Protein Names: | A20-binding inhibitor of NF-kappa-B activation 1; ABIN-1; HIV-1 Nef-interacting protein; Nef-associated factor 1; Naf1; Nip40-1; Virion-associated nuclear shuttling protein; VAN; hVAN |
| Protein Family: | TNFAIP3-interacting protein |
| UniProt Gene Name: | TNIP1Â Â |
| UniProt Entry Name: | TNIP1_HUMAN |
| Antibodies |
| TNIP1 Antibody (PACO12829) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
