The TNNI3 Antibody (CAB0152) is a high-quality antibody developed for reliable detection and analysis of target proteins. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19. RRID AB_2756984 Gene ID 7137 Swiss Prot Synonym CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; Cardiac Troponin I (TNNI3)
This antibody is validated for use in WB, IP, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
TNNI3 Antibody
SKU:
CAB0152
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CMH7, RCM1, cTnI, CMD2A, TNNC1, CMD1FF, Cardiac Troponin I (TNNI3)
Positive Sample:
Mouse heart, Rat lung
Cellular Localization:
Cardiac Myofibril, Cytosol, Sarcomere.
Calculated MW:
24kDa
Observed MW:
28kDa
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19. RRID AB_2756984 Gene ID 7137 Swiss Prot Synonym CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; Cardiac Troponin I (TNNI3)
Purification Method:
Affinity purification
Gene ID:
7137
RRID:
AB_2756984
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse heart, using Cardiac Troponin I (TNNI3) Rabbit pAb (CAB0152) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Western blot analysis of lysates from Rat lung, using Cardiac Troponin I (TNNI3) Rabbit pAb (CAB0152) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunoprecipitation analysis of 600 μg extracts of Mouse heart cells using 3 μg Cardiac Troponin I (TNNI3) antibody (CAB0152). Western blot was performed from the immunoprecipitate using Cardiac Troponin I (TNNI3) antibody (CAB0152) at a dilution of 1:1000.
