The TRADD Antibody (CAB1145) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications.TRADD, or TNFRSF1A-associated via death domain, is a crucial mediator of cell death signaling pathways, particularly in response to tumor necrosis factor (TNF) receptor activation. Understanding the role of TRADD in cell death pathways is essential for unraveling the molecular mechanisms underlying conditions such as cancer, inflammation, and neurodegenerative diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
TRADD Antibody
SKU:
CAB1145
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
9130005N23Rik, TRADD
Positive Sample:
MCF7
Cellular Localization:
Cytoplasm, Nucleus, Cytoskeleton.
Calculated MW:
34kDa
Observed MW:
36kDa
Predicted to enable death domain binding activity; identical protein binding activity; and signaling receptor binding activity. Involved in positive regulation of hair follicle development. Acts upstream of or within extrinsic apoptotic signaling pathway. Located in cytoplasm. Is expressed in adrenal gland; genitourinary system; liver; lung; and spleen. Orthologous to human TRADD (TNFRSF1A associated via death domain).
Purification Method
Affinity purification
Gene ID
8717
RRID
AB_2758565
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from MCF7 cells, using TRADD Rabbit pAb (CAB1145) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
