The TRAF2 Monoclonal Antibody (CAB19129) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, is highly specific for human samples and has been validated for use in Western blotting applications. By targeting the TRAF2 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for research in immunology and cancer biology.TRAF2 is a critical component of the tumor necrosis factor receptor (TNFR) signaling pathway, and its dysregulation has been implicated in various disease states, including cancer, inflammatory disorders, and autoimmune diseases.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRAF2 Monoclonal Antibody
SKU:
CAB19129
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC51292
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.
Purification Method
Affinity purification
Gene ID
7186
RRID
AB_2862622
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KD Validated] TRAF2 Rabbit mAb (CAB19129) at 1:50000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates, using [KD Validated] TRAF2 Rabbit mAb (CAB19129) at 1:50000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg TRAF2 antibody (CAB19129). Western blot was performed from the immunoprecipitate using TRAF2 antibody (CAB19129) at a dilution of 1:50000.
