The TRAP1 Antibody (CAB4378) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit immunization, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for Western blot applications. By specifically binding to TRAP1 protein, this antibody allows for precise detection and analysis in various cell types, facilitating studies in mitochondrial biology and cancer research.TRAP1, also known as tumor necrosis factor type 1 receptor-associated protein, is a crucial player in maintaining mitochondrial function and protecting cells from stress-induced damage.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRAP1 Antibody
SKU:
CAB4378
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10131
RRID
AB_2765649
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TRAP1 Rabbit pAb (CAB4378) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using TRAP1 Rabbit pAb (CAB4378) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using TRAP1 Rabbit pAb (CAB4378) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using TRAP1 Rabbit pAb (CAB4378) at dilution of 1:100. Blue: DAPI for nuclear staining.
