The TRH Antibody (CAB14472) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it suitable for a variety of experimental applications.TRH is a neurotransmitter that plays a key role in the regulation of the hypothalamic-pituitary-thyroid axis, controlling the release of thyroid-stimulating hormone (TSH) from the pituitary gland. The TRH receptor is a G protein-coupled receptor that mediates the effects of TRH in various physiological processes, including the regulation of metabolism, energy balance, and stress responses.With its ability to bind to the TRH receptor protein, the CAB14472 antibody enables the detection and analysis of TRH receptor expression in different cell types and tissues.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRH Antibody
SKU:
CAB14472
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TRF, Pro-TRH, TRH
Positive Sample:
U-87MG, BxPC-3, A-549, DU145, Mouse brain, Rat testis
Cellular Localization:
Secreted.
Calculated MW:
27kDa
Observed MW:
27kDa
This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism.
Purification Method
Affinity purification
Gene ID
7200
RRID
AB_2761344
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TRH Rabbit pAb (CAB14472) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using TRH Rabbit pAb (CAB14472) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of H9C2 cells using TRH Rabbit pAb (CAB14472) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
