The TRIM32 Antibody (CAB7079) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting Trim32 in human samples, making it ideal for Western blot applications.Trim32, also known as tripartite motif-containing protein 32, has been implicated in a variety of cellular functions, such as muscle differentiation and repair, as well as tumorigenesis. By targeting Trim32 with this polyclonal antibody, researchers can investigate its role in different cell types and gain insights into its potential implications in diseases such as cancer and muscular dystrophy.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRIM32 Antibody
SKU:
CAB7079
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HT2A, BBS11, TATIP, LGMD2H, LGMDR8, TRIM32
Positive Sample:
HeLa, Mouse testis, mouse brain, rat testis, rat brain
Cellular Localization:
Cytoplasm.
Calculated MW:
72kDa
Observed MW:
75kDa
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.
Purification Method
Affinity purification
Gene ID
22954
RRID
AB_2767634
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TRIM32 Rabbit pAb (CAB7079) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from HeLa cells, using TRIM32 Rabbit pAb (CAB7079) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
