The Trim54 Polyclonal Antibody (CAB13111) is a valuable tool for researchers studying Trim54, a protein involved in the regulation of muscle fiber size and function. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By binding specifically to Trim54, this antibody enables the detection and analysis of the protein in various cell types, making it an essential component for studies in muscle biology and related fields.
Trim54 is known for its role in muscle development and function, particularly in the regulation of muscle fiber size and contractile properties. Understanding the function of Trim54 is important for research into muscle diseases, muscle regeneration, and muscle wasting conditions. The Trim54 Polyclonal Antibody offers researchers a reliable tool for investigating the mechanisms by which Trim54 influences muscle physiology, providing valuable insights for therapeutic interventions in muscle-related disorders.
Product Name:
TRIM54 Rabbit Polyclonal Antibody
SKU:
CAB13111
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 179-358 of human TRIM54 (NP_912730.2).
Cytoplasm, Z line, cytoskeleton, myofibril, sarcomere.
Calculated MW:
40kDa
Observed MW:
40kDa
The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Purification Method:
Affinity purification
Gene ID:
57159
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TRIM54 Rabbit pAb (CAB13111) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
