Anti-TRIM69 Antibody (CAB5910)

Antibody Name:	Anti-TRIM69 Antibody
Antibody SKU:	CAB5910
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human TRIM69 (NP_542783.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse testis, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human TRIM69 (NP_542783.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEEE LAIQ QGQL ETTL KELQ TLRN MQKE AIAA HKEN KLHL QQHV SMEF LKLH QFLH SKEK DILT ELRE EGKA LNEE MELN LSQL QEQC LLAK DMLV SIQA KTEQ QNSF DFLK DITT LLHS LEQG MKVL ATRE LISR KLNL GQYK GPIQ YMVW REMQ DTLC PGLS PLTL DPKT AHPN LVLS KSQT SVWH GDIK KIMP DDPE RFDS SVAV LGSR GFTS GKWY WEVE VAKK TKWT VGVV RESI IRKG SCPL TPEQ GFWL LRLR NQTD LKAL DLPS FSLT LTNN LDKV GIYL DYEG GQLS FYNA KTMT HIYT FSNT FMEK LYPY FCPC LNDG GENK EPLH ILHP Q
Gene ID:	140691
Uniprot:	Q86WT6
Cellular Location:	Cytoplasm, Nucleus, Nucleus speckle
Calculated MW:	32kDa/34kDa/39kDa/57kDa
Observed MW:	60KDa

Synonyms:

TRIM69, HSD-34, HSD34, RNF36, Trif

Background:

This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.

UniProt Protein Function:	TRIM69: May play a role in apoptosis. Belongs to the TRIM/RBCC family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 15q21.1 Cellular Component: cytoplasm; cytosol; nuclear speck; nucleus; plasma membrane Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: protein polyubiquitination
NCBI Summary:	This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q86WT6
NCBI GenInfo Identifier:	229462760
NCBI Gene ID:	140691
NCBI Accession:	Q86WT6.2
UniProt Secondary Accession:	Q86WT6,Q309B0, Q4G1A5, Q6W897, Q8IYY3, Q8WY16, Q8WY17 A8MX03,
UniProt Related Accession:	Q86WT6
Molecular Weight:	32,068 Da
NCBI Full Name:	E3 ubiquitin-protein ligase TRIM69
NCBI Synonym Full Names:	tripartite motif containing 69
NCBI Official Symbol:	TRIM69
NCBI Official Synonym Symbols:	Trif; HSD34; RNF36; HSD-34
NCBI Protein Information:	E3 ubiquitin-protein ligase TRIM69
UniProt Protein Name:	E3 ubiquitin-protein ligase TRIM69
UniProt Synonym Protein Names:	RFP-like domain-containing protein trimless; RING finger protein 36; Tripartite motif-containing protein 69
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	TRIM69
UniProt Entry Name:	TRI69_HUMAN

	Western blot analysis of extracts of various cell lines, using TRIM69 antibody (CAB5910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human kidney using TRIM69 antibody (CAB5910) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using TRIM69 antibody (CAB5910). Blue: DAPI for nuclear staining.

Antibodies

TRIM69 Antibody (PACO12924)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)