The TRIM71 Polyclonal Antibody (PAC020703) is a valuable tool for researchers studying TRIM71, a protein involved in RNA degradation and gene regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications including Western blot and immunohistochemistry.TRIM71, also known as Lin41, is a key player in post-transcriptional gene silencing and has been implicated in regulating stem cell differentiation and development. Its role in controlling gene expression makes it a potential target for research in fields such as stem cell biology, developmental biology, and cancer research.
By using the TRIM71 Polyclonal Antibody, researchers can accurately detect and analyze TRIM71 expression in different cell types and tissues, providing valuable insights into its functions and potential therapeutic applications. This antibody is a valuable tool for advancing our understanding of TRIM71's role in various biological processes and disease states.
Antibody Name:
TRIM71 Antibody (PACO20703)
Antibody SKU:
PACO20703
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:1000-1:5000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide of human TRIM71
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE,Lysate: 40 μgPrimary antibody: PACO20703(TRIM71 Antibody) at dilution 1/300 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 20 seconds.
Background:
LIN-41, also called tripartite motif-containing 71 (TRIM71), which was first identified in Caenorhabditis elegans, is responsible for the timing of cell fate determination. By encoding microRNAs (miRNAs), the heterochronic genes let-7 and lin-4 downregulate the gene encoding LIN-41. The miRNAs bind to six complementary sites on the 3' untranslated region (UTR) of the LIN-41 gene. This downregulation positively regulates the timing of the expression of LIN-29, an adult specification transcription factor.
Synonyms:
tripartite motif containing 71, E3 ubiquitin protein ligase
UniProt Protein Function:
TRIM71: May be involved in controlling the timing of organ formation during development. Belongs to the TRIM/RBCC family.Chromosomal Location of Human Ortholog: 3p22.3Molecular Function: miRNA binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activityBiological Process: neural tube development; protein autoubiquitination; fibroblast growth factor receptor signaling pathway; neural tube closure; miRNA-mediated gene silencing, negative regulation of translation; G1/S transition of mitotic cell cycle
