The TriMethyl-Histone H4-K20 Antibody (CAB2372) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to the trimethylated form of histone H4 at lysine 20 and is suitable for use in a variety of applications, including Western blot and immunohistochemistry.Histone H4 lysine 20 trimethylation is a crucial epigenetic mark associated with gene repression and chromatin compaction. Dysregulation of this modification has been linked to various diseases, including cancer and developmental disorders. The CAB2372 antibody enables precise detection and analysis of trimethyl histone H4 (K20) levels in different cell types and tissues, making it an essential tool for epigenetic research.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA, DB applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
TriMethyl-Histone H4-K20 Antibody
SKU:
CAB2372
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
HRKV L
Tested Applications:
WBIHC-PIF/ICCELISADB
Recommended Dilution:
WB
1:500 - 1:2000
DB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method
Affinity purification
Gene ID
8359
RRID
AB_2764332
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using TriMethyl-Histone H4-K20 Rabbit pAb (CAB2372) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H4-K20 antibody (CAB2372).
Immunohistochemistry analysis of paraffin-embedded Mouse testis using TriMethyl-Histone H4-K20 Rabbit pAb (CAB2372) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Rabbit pAb (CAB2372). Blue: DAPI for nuclear staining.
Giannopoulou, A.I et al.
LINE-1 hypomethylation in cell-free DNA of high-grade glioma patients correlates with tissue levels and is associated with reduced DNMT1 and H4K20me3 expression
