The TRMT2B Antibody (CAB3509) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity and sensitivity for detecting TRMT2B in human samples, making it suitable for use in Western blot applications. By targeting the TRMT2B protein, this antibody allows for the precise detection and analysis of TRMT2B expression in a variety of cell types, making it ideal for investigations in molecular biology and RNA processing.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
TRMT2B Antibody
SKU:
CAB3509
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CXorf34, dJ341D10.3, TRMT2B
Positive Sample:
Raji, U-87MG, SW480, MCF7, NCI-H460
Cellular Localization:
Mitochondrion.
Calculated MW:
56kDa
Observed MW:
45kDa
This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms.
Purification Method
Affinity purification
Gene ID
79979
RRID
AB_2765137
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TRMT2B Rabbit pAb (CAB3509) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
