The TRMT61B Antibody (CAB8067) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the TRMT6/61B protein complex, this antibody allows for precise detection and analysis in a variety of cell types, making it well-suited for investigations in molecular biology and RNA biology.TRMT6/61B is known for its role in catalyzing the methylation of tRNAs, a process crucial for proper tRNA function and protein synthesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TRMT61B Antibody
SKU:
CAB8067
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TRMT61B
Positive Sample:
Mouse liver
Cellular Localization:
Mitochondrion.
Calculated MW:
53kDa
Observed MW:
52kDa
Enables mRNA (adenine-N1-)-methyltransferase activity; rRNA (adenine) methyltransferase activity; and tRNA (adenine-N1-)-methyltransferase activity. Involved in mRNA methylation; mitochondrial tRNA methylation; and protein homooligomerization. Located in mitochondrion.
Purification Method
Affinity purification
Gene ID
55006
RRID
AB_2772724
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using TRMT61B Rabbit pAb (CAB8067) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
