The TRNT1 Antibody (CAB17699) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and is optimized for Western blot applications. By binding specifically to the TRNT1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and RNA processing research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TRNT1 Antibody
SKU:
CAB17699
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CCA1, RPEM, SIFD, MtCCA, CGI-47, TRNT1
Positive Sample:
HeLa, A-549, Mouse kidney, Rat spleen, Rat lung, Rat brain
Cellular Localization:
Mitochondrial Matrix, Mitochondrion, Nucleoplasm.
Calculated MW:
50kDa
Observed MW:
50kDa
The protein encoded by this gene is a CCA-adding enzyme which belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. This essential enzyme functions by catalyzing the addition of the conserved nucleotide triplet CCA to the 3' terminus of tRNA molecules. Mutations in this gene result in sideroblastic anemia with B-cell immunodeficiency, periodic fevers, and developmental delay. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
51095
RRID
AB_2772725
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TRNT1 Rabbit pAb (CAB17699) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
