The TSEN54 Antibody (CAB15227) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically designed to target the TSEN54 protein, an essential component of the tRNA splicing endonuclease complex.TSEN54 plays a crucial role in the processing of precursor tRNAs, ensuring accurate splicing and maturation of these essential molecules. By targeting TSEN54, researchers can investigate the mechanisms underlying RNA splicing and its impact on gene expression and cellular function.The TSEN54 Polyclonal Antibody is highly reactive with human samples and has been validated for use in Western blot applications.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TSEN54 Antibody
SKU:
CAB15227
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PCH4, PCH5, PCH2A, sen54, SEN54L, TSEN54
Positive Sample:
HeLa, MCF7, 293T, Mouse kidney, Rat kidney
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
59kDa
Observed MW:
59kDa
This gene encodes a subunit of the tRNA splicing endonuclease complex, which catalyzes the removal of introns from precursor tRNAs. The complex is also implicated in pre-mRNA 3-prime end processing. Mutations in this gene result in pontocerebellar hypoplasia type 2.
Purification Method
Affinity purification
Gene ID
283989
RRID
AB_2762121
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TSEN54 Rabbit pAb (CAB15227) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
