The TSPAN9 Polyclonal Antibody (PACO57004) is a valuable tool for researchers studying the cell surface protein TSPAN9, which plays a key role in various biological processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to TSPAN9, this antibody enables precise detection and analysis of the protein in different cell types, making it essential for studies in cell biology and cancer research.TSPAN9 is involved in cell signaling, migration, and proliferation, making it a crucial target for investigation in various diseases, including cancer and developmental disorders.
Its role in modulating cellular processes makes it a promising candidate for therapeutic interventions aimed at targeting specific pathways implicated in disease progression. Research using the TSPAN9 Polyclonal Antibody can provide valuable insights into the mechanisms underlying these conditions and potentially lead to the development of novel treatment strategies.
IHC image of PACO57004 diluted at 1:700 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO57004 at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO57004 diluted at 1:700 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
focal adhesion, integral component of plasma membrane, plasma membrane, tetraspanin-enriched microdomain, cell surface receptor signaling pathway
TSPAN9: is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. Alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq, Nov 2009]Protein type: Membrane protein, integral; Membrane protein, multi-passChromosomal Location of Human Ortholog: 12p13.33-p13.32Cellular Component: focal adhesion; integral to plasma membrane; plasma membraneBiological Process: cell surface receptor linked signal transduction
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. Alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq, Nov 2009]
