The UBAsh3A Polyclonal Antibody (PAC022637) is a valuable tool for research involving the UBAsh3A protein, which is involved in various cellular processes related to ubiquitin signaling. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.UBAsh3A is a key player in the regulation of ubiquitin-mediated events, including protein degradation, DNA repair, and cellular signaling pathways. Its role in these processes makes it a promising target for studies in molecular biology, cancer research, and neurodegenerative diseases.
By using the UBAsh3A Polyclonal Antibody, researchers can detect and analyze UBAsh3A protein levels in different cell types, allowing for a deeper understanding of its functions and mechanisms of action. This antibody is essential for investigating the intricate network of ubiquitin signaling and its implications in various physiological and pathological conditions.
Antibody Name:
UBASH3A Antibody (PACO22637)
Antibody SKU:
PACO22637
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human UBASH3A.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from LOVO cells, HuvEc cells and K562 cells, using UBASH3A antibody.
Background:
Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.
Synonyms:
Suppressor of T-cell receptor signaling 2; STS-2; Cbl-interacting protein 4; CLIP4; T-cell ubiquitin ligand
UniProt Protein Function:
STS2: Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Protein phosphatase, tyrosine (non-receptor)Chromosomal Location of Human Ortholog: 21q22.3Cellular Component: cytosolMolecular Function: protein binding
UniProt Protein Details:
NCBI Summary:
This gene encodes one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. Both family members can negatively regulate T-cell signaling. This family member can facilitate growth factor withdrawal-induced apoptosis in T cells, which may occur via its interaction with AIF, an apoptosis-inducing factor. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]
