The UBE2G2 Antibody (CAB10408) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it ideal for use in Western blot applications. By specifically binding to the UBE2G2 protein, researchers can easily detect and analyze its expression in a variety of cell types, facilitating studies in the fields of molecular biology and cancer research.UBE2G2 is a crucial enzyme involved in the regulation of protein degradation through the ubiquitin-proteasome system, making it a potential target for therapeutic interventions in diseases such as cancer and neurodegenerative disorders.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
UBE2G2 Antibody
SKU:
CAB10408
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UBC7, UBE2G2
Positive Sample:
MCF7, DU145, HT-1080, Mouse heart, Mouse testis, Rat heart, Rat testis
Cellular Localization:
Cytosol, Endoplasmic Reticulum, Lipid Droplet.
Calculated MW:
19kDa
Observed MW:
19kDa
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse counterpart. This gene is ubiquitously expressed, with high expression seen in adult muscle. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
7327
RRID
AB_2757956
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using UBE2G2 Rabbit pAb (CAB10408) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of NIH-3T3 cells using UBE2G2 Rabbit pAb (CAB10408) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
