The UBE2NL Antibody (CAB8381) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the UBE2NL protein, this antibody enables detection and analysis in various cell types, making it an ideal tool for studies in molecular biology, cell signaling, and protein degradation pathways.UBE2NL is a crucial component in the ubiquitin-proteasome system, which controls the degradation of damaged or unwanted proteins in the cell.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
UBE2NL Antibody
SKU:
CAB8381
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Li174, UBE2NL
Positive Sample:
HL-60, Mouse brain, Mouse testis, Mouse liver
Cellular Localization:
Extracellular Exosome, Nucleus.
Calculated MW:
17kDa
Observed MW:
17kDa
This gene is intronless and encodes a member of the ubiquitin-conjugating enzyme family. The protein product is 91% identical to ubiquitin-conjugating enzyme E2N, a multi-exon gene product. This locus represents a polymorphic pseudogene, where some individuals contain an allele that can encode a full-length protein, while others have a non-functional allele containing a premature stop codon (reference SNP rs237520) that truncates the coding sequence.
Purification Method
Affinity purification
Gene ID
389898
RRID
AB_2772780
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using UBE2NL Rabbit pAb (CAB8381) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
