The UBR5 Antibody (CAB13816) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. RRID AB_2760671 Gene ID 51366 Swiss Prot Synonym DD5; EDD; HYD; EDD1; UBR5
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
UBR5 Antibody
SKU:
CAB13816
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DD5, EDD, HYD, EDD1, UBR5
Positive Sample:
U-87MG, 293T, Jurkat, HeLa
Cellular Localization:
Nucleus.
Calculated MW:
309kDa
Observed MW:
300kDa
This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. RRID AB_2760671 Gene ID 51366 Swiss Prot Synonym DD5; EDD; HYD; EDD1; UBR5
Purification Method:
Affinity purification
Gene ID:
51366
RRID:
AB_2760671
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using UBR5 Rabbit pAb (CAB13816) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
