The PGP9.5/UCHL1 Antibody (CAB2131) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards UCHL1 in human samples, making it ideal for use in Western blotting and immunohistochemistry applications.UCHL1, also known as ubiquitin C-terminal hydrolase L1, is involved in the removal of abnormal or misfolded proteins, playing a crucial role in maintaining cellular homeostasis. Dysregulation of UCHL1 has been linked to neurodegenerative disorders such as Parkinson's and Alzheimer's disease, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PGP9.5/UCHL1 Antibody
SKU:
CAB2131
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
Purification Method
Affinity purification
Gene ID
7345
RRID
AB_2764150
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PGP9.5/UCHL1 Rabbit pAb (CAB2131) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of C6 cells using PGP9.5/PGP9.5/UCHL1 Rabbit pAb (CAB2131) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
