The UFSP2 Polyclonal Antibody (PAC041986) is a valuable tool for researchers studying UFSP2, a protein involved in the regulation of cellular processes such as DNA repair and oxidative stress response. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to UFSP2, researchers can effectively detect and analyze the protein in various cell types, making it a versatile option for studies in molecular biology and disease mechanisms.
UFSP2, also known as Ubiquitin-fold Modifier Conjugating Enzyme 1 (Ufm1) Specific Protease 2, is a key player in maintaining cellular homeostasis by ensuring proper protein modification and turnover. Dysregulation of UFSP2 has been linked to various diseases, including cancer and neurodegenerative disorders, emphasizing its importance in cellular function. Investigating the role of UFSP2 can provide valuable insights into disease pathogenesis and potential therapeutic targets for intervention.
Western blot. All lanes: UFSP2 antibody at 4µg/ml. Lane 1: HepG2 whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 54 kDa. Observed band size: 54 kDa.
Immunofluorescent analysis of Hela cells using PACO41986 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO41986 at dilution of 1:100.
Background:
Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of UFM1, a ubiquitin-like modifier protein bound to a number of target proteins. Does not hydrolyze SUMO1 or ISG15 ubiquitin-like proteins. Through TRIP4 deufmylation may regulate intracellular nuclear receptors transactivation and thereby regulate cell proliferation and differentiation.
UFSP2: Thiol protease which recognizes and hydrolyzes the peptide bond at the C-terminal Gly of UFM1, an ubiquitin-like modifier protein bound to a number of target proteins. Does not hydrolyze SUMO1 or ISG15 ubiquitin-like proteins. Belongs to the peptidase C78 family.Protein type: Protease; EC 3.4.22.-Chromosomal Location of Human Ortholog: 4q35.1Cellular Component: endoplasmic reticulum; cytoplasm; nucleusMolecular Function: protein binding; thiolester hydrolase activityBiological Process: regulation of estrogen receptor signaling pathway; proteolysisDisease: Beukes Hip Dysplasia
UniProt Protein Details:
NCBI Summary:
Like ubiquitin (see MIM 191339), ubiquitin-fold modifier-1 (UFM1; MIM 610553) must be processed by a protease before it can conjugate with its target proteins. UFSP2 is a thiol protease that specifically processes the C terminus of UFM1 (Kang et al., 2007 [PubMed 17182609]).[supplied by OMIM, Oct 2009]
