The UGT1A4 Polyclonal Antibody (CAB5549) is a valuable tool for researchers studying the UGT1A4 enzyme, which plays a crucial role in drug metabolism and detoxification processes in the liver. This antibody, raised in rabbits, offers high specificity and reactivity with human samples, making it ideal for Western blot applications.UGT1A4 is a key member of the UDP-glucuronosyltransferase enzyme family, responsible for catalyzing the conjugation of a variety of endogenous and exogenous compounds with glucuronic acid, facilitating their elimination from the body.
Its involvement in drug metabolism pathways highlights its importance in pharmacology and toxicology research.Researchers studying drug-drug interactions, pharmacokinetics, and drug efficacy can benefit from the use of the UGT1A4 Polyclonal Antibody in their experiments. By detecting and quantifying UGT1A4 protein levels in various tissues and cell types, this antibody enables accurate analysis and investigation into the role of this enzyme in drug metabolism and detoxification pathways.
Product Name:
UGT1A4 Rabbit Polyclonal Antibody
SKU:
CAB5549
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 29-150 of human UGT1A4 (NP_009051.1).
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. This enzyme has some glucuronidase activity towards bilirubin, although is is more active on amines, steroids, and sapogenins.
Purification Method:
Affinity purification
Gene ID:
54657
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using UGT1A4 antibody (CAB5549) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
