The USP12 Antibody (CAB17862) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting USP12 in human samples, making it ideal for Western blot applications. By binding to the USP12 protein, this antibody enables precise detection and analysis in a range of cell types, facilitating research in fields such as biochemistry and cell biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
USP12 Antibody
SKU:
CAB17862
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MEIL MTVS KFAS ICTM GANA SALE KEIG PEQF PVNE HYFG LVNF GNTC YCNS VLQA LYFC RPFR EKVL AYKS QPRK KESL LTCL ADLF HSIA TQKK KVGV
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UBH1, USP12L1, USP12
Positive Sample:
A-375, A-549, mouse brain
Cellular Localization:
Cytosol, Nucleoplasm, Nucleus.
Calculated MW:
43kDa
Observed MW:
42kDa
Enables cysteine-type endopeptidase activity and thiol-dependent deubiquitinase. Involved in protein deubiquitination. Predicted to be located in nucleoplasm. Predicted to be active in cytosol and nucleus.
Purification Method
Affinity purification
Gene ID
219333
RRID
AB_2772820
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using USP12 Rabbit pAb (CAB17862) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 2min.
