The USP16 Antibody (CAB5861) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has high reactivity specifically with human samples, making it ideal for Western blot applications. By targeting the USP16 protein, this antibody allows for precise detection and analysis in various cell types, facilitating research in areas such as cell biology, cancer biology, and drug development.USP16, also known as ubiquitin-specific protease 16, plays a crucial role in controlling the stability and function of various proteins involved in cell proliferation, differentiation, and DNA repair.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Monkey samples.
Product Name:
USP16 Antibody
SKU:
CAB5861
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Monkey
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UBPM, UBP-M, USP16
Positive Sample:
Mouse testis, Rat testis
Cellular Localization:
Nucleus.
Calculated MW:
94kDa
Observed MW:
110kDa
This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method
Affinity purification
Gene ID
10600
RRID
AB_2766611
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using USP16 Rabbit pAb (CAB5861) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
