The USP25 Antibody (CAB12588) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the USP25 protein, this antibody allows for the detection and analysis of USP25 expression in a variety of cell types, making it ideal for studies in molecular biology and cell signaling pathways.USP25 is known for its involvement in diverse cellular functions, such as DNA repair, cell cycle regulation, and protein turnover.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
USP25 Antibody
SKU:
CAB12588
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
USP21, USP25
Positive Sample:
293F, U-251MG, RAW264.7, Mouse liver, PC-12
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
122kDa
Observed MW:
130kDa
Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).
Purification Method
Affinity purification
Gene ID
29761
RRID
AB_2759430
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using USP25 Rabbit pAb (CAB12588) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
