V5-Tag Monoclonal Antibody is a premium monoclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IF, IP, FC, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with All samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ul format to meet your experimental needs. For optimal performance, store at Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
V5-Tag Monoclonal Antibody (MACO0669)
SKU:
MACO0669
Size:
50μl
Isotype:
IgG1
Host Species:
Mouse
Reactivity:
All
Immunogen:
GKPIPNPLLGLDST synthetic peptide conjugate to KLH
Form:
Liquid
Tested Applications:
ELISAWBIFIPFC
Recommended Dilution:
WB 1:2000-1:80000, IF 1:100-1:200, IP 2µl-5µl, FC 1:100-1:300
Western Blot Positive WB detected in: 50ng V5-tagged fusion protein V5 Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 37.8 KDa Observed band size: 43 KDa Exposure time: 10S
Western Blot Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:5000 Lane 1: Recombinant V5-tagged fusion protein at 50ng Lane 2: Recombinant V5-tagged fusion protein at 25ng Lane 3: Recombinant V5-tagged fusion protein at 12.25ng Lane 4: Recombinant V5-tagged fusion protein at 6.25ng Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 37.8 kd Observed band size: 43 kd Exposure time: 5min
Western Blot Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4 at 50ng All lanes: V5 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 50.2, 47.6, 66.7, 37.8 KDa Observed band size: 52, 47, 67, 42 KDa Exposure time: 30s
Western Blot Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, at 50ng All lanes: V5 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 31.9, 20.4, 24.2, 53.4 KDa Observed band size: 32, 21, 28, 58 KDa Exposure time: 5min
Immunofluorescence staining of transfected HEK293F cells with MACO0669 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).
Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate Lane 1: Mouse control IgG1 instead of MACO0669 in Transfected HEK293F cells whole cell lysate Lane 2: MACO0669 (2.5µl) + Transfected HEK293F cells whole cell lysate (500µg) Lane 3: Transfected HEK293F cells whole cell lysate (20µg) For western blotting, the blot was detected with MACO0669 at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
Overlay histogram showing untransfected HEK293F cells (green line) or transfected HEK293F cells (red line) stained with MACO0669. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1:200/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Acquisition of >10,000 events was performed.
