The VAMP2 Antibody (CAB1249) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit immunization, is highly specific to human VAMP2 and has been validated for use in Western blot applications. By binding to the VAMP2 protein, this antibody enables accurate detection and analysis of VAMP2 expression in various cell types, making it an ideal choice for studies in neuroscience and neurology research.VAMP2 plays a crucial role in synaptic transmission by mediating the fusion of neurotransmitter-containing vesicles with the plasma membrane.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
VAMP2 Antibody
SKU:
CAB1249
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SYB2, VAMP-2, NEDHAHM, VAMP2
Positive Sample:
22Rv1, Mouse brain, Mouse lung, Rat brain
Cellular Localization:
Cell Junction, Cell Membrane, Cytoplasmic Vesicle, Single-Pass Type Iv Membrane Protein, Secretory Vesicle, Synapse, Synaptic Vesicle Membrane, Synaptosome.
Calculated MW:
13kDa
Observed MW:
16kDa
The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG.
Purification Method
Affinity purification
Gene ID
6844
RRID
AB_2759332
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using VAMP2 Rabbit pAb (CAB1249) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using VAMP2 Rabbit pAb (CAB1249) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using VAMP2 Rabbit pAb (CAB1249) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
