The VAMP4 Antibody (CAB4241) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the VAMP4 protein, this antibody enables the detection and analysis of VAMP4 in various cell types, making it an excellent choice for studies in cell biology and neuroscience.VAMP4, also known as vesicle-associated membrane protein 4, plays a critical role in intracellular vesicle trafficking and fusion processes. Its involvement in these fundamental cellular processes makes it a key player in diverse biological functions, including neurotransmitter release, hormone secretion, and organelle dynamics.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
VAMP4 Antibody
SKU:
CAB4241
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VAMP-4, VAMP24, VAMP4
Positive Sample:
B cells, HeLa, Jurkat, BT-474, NIH/3T3, Mouse brain, Mouse lung, Rat brain
Cellular Localization:
Golgi Apparatus, Single-Pass Type Iv Membrane Protein, Trans-Golgi Network Membrane.
Calculated MW:
16kDa
Observed MW:
16kDa
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport.
Purification Method
Affinity purification
Gene ID
8674
RRID
AB_2765579
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using VAMP4 Rabbit pAb (CAB4241) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
