The VEGFA Polyclonal Antibody (AB10001) is a key tool for researchers studying Vascular Endothelial Growth Factor A (VEGFA), a protein involved in angiogenesis, vasculogenesis, and endothelial cell growth. This antibody, produced in rabbits, demonstrates high specificity and sensitivity for detecting VEGFA in human samples, making it an essential tool for studies in cancer biology, cardiovascular diseases, and developmental biology.VEGFA is a crucial regulator of blood vessel formation and is implicated in various physiological and pathological processes, including tumor growth and metastasis. By targeting VEGFA with this antibody, researchers can analyze the expression levels and localization of VEGFA in different tissues and cell types, providing valuable insights into the role of VEGFA in disease progression and potential therapeutic interventions.
Furthermore, the VEGFA Polyclonal Antibody is validated for use in various applications, including immunohistochemistry, immunofluorescence, and ELISA, offering researchers flexibility in their experimental design. Its performance in these techniques ensures reliable and reproducible results, making it an indispensable tool for investigating the intricate mechanisms of VEGFA-mediated signaling pathways and its implications in disease pathology.
Product Name:
Vegfa Polyclonal Antibody
SKU:
CAB22678
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant protein of mouse Vegfa.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
Vpf; Vegf; Vegfa
Positive Sample:
Mouse heart
Conjugate:
Unconjugated
Calculated MW:
22kDa
Observed MW:
26kDa
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.
Purification Method:
Affinity purification
Gene ID:
22339
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of Mouse heart, using Vegfa antibody (CAB22678) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.8s.
