The Vitamin K1 ELISA Kit from Assay Genie is specifically designed for the precise measurement of Vitamin K1 levels in various biological samples. With high sensitivity and specificity, this kit ensures accurate and reliable results, making it a valuable tool for research in the fields of nutrition, cardiovascular health, and bone metabolism.Vitamin K1, also known as phylloquinone, is a vital nutrient necessary for proper blood clotting and bone health. Deficiencies in Vitamin K1 have been associated with an increased risk of cardiovascular diseases, osteoporosis, and other health issues.
By measuring Vitamin K1 levels, researchers can better understand its role in disease development and potentially develop targeted therapies to address these conditions.Overall, the Vitamin K1 ELISA Kit from Assay Genie provides researchers with a powerful tool to study the importance of Vitamin K1 in various physiological processes and disease states, ultimately leading to advancements in preventive and therapeutic strategies.
Product Name:
VK1 (Vitamin K1) ELISA Kit
SKU:
UNES00014
Target:
VK1 (Vitamin K1) (UNES00014)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VK1. During the reaction, VK1 in the sample or standard competes with a fixed amount of VK1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VK1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VK1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
97-112
95-109
Average (%)
104
102
103
1:4
Range (%)
93-108
96-113
95-109
Average (%)
101
103
102
1:8
Range (%)
95-111
93-105
94-106
Average (%)
101
99
100
1:16
Range (%)
99-115
95-113
92-103
Average (%)
105
103
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
84-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.2
94.73
361.53
43.96
94.7
340.66
Standard deviation
2.92
4.09
18.08
2.59
3.94
17.31
C V (%)
6.32
4.32
5.0
5.89
4.16
5.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of VK1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes VK1 in samples. No significant cross-reactivity or interference between VK1 and analogues was observed.
