Von Hippel Lindau/VHL Monoclonal Antibody (CAB23239)
The Von Hippel Lindau/VHL Monoclonal Antibody (CAB23239) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in mice, has high specificity for human samples and has been extensively validated for use in various applications, including immunohistochemistry and flow cytometry.The VHL protein is known for its role in the development of von Hippel-Lindau disease, a rare genetic disorder characterized by the formation of tumors in various organs.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Von Hippel Lindau/VHL Monoclonal Antibody
SKU:
CAB23239
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC59984
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RCA1, VHL1, pVHL, HRCA1, Von Hippel Lindau/VHL
Positive Sample:
HeLa, 293T, Mouse brain, Mouse kidney, Rat brain, Rat kidney
This gene encodes a component of a ubiquitination complex. The encoded protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. In addition to oxygen-related gene expression, this protein plays a role in many other cellular processes including cilia formation, cytokine signaling, regulation of senescence, and formation of the extracellular matrix. Variants of this gene are associated with von Hippel-Lindau syndrome, pheochromocytoma, erythrocytosis, renal cell carcinoma, and cerebellar hemangioblastoma.
Purification Method
Affinity purification
Gene ID
7428
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KO Validated] Von Hippel Lindau/VHL Rabbit mAb (CAB23239) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Western blot analysis of lysates from wild type (WT) and Von Hippel Lindau/VHL knockout (KO) 293T cells using [KO Validated] Von Hippel Lindau/VHL Rabbit mAb (CAB23239) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
