The VPS35 Monoclonal Antibody (CAB9278) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated from rabbit cells, exhibits high specificity and sensitivity towards VPS35 in human samples, making it an essential tool for Western blot applications. By binding to the VPS35 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in cell biology and neurodegenerative diseases.VPS35 is crucial for maintaining cellular homeostasis by regulating the recycling of proteins from endosomes to the trans-Golgi network, impacting cell signaling, vesicle trafficking, and autophagy.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
VPS35 Monoclonal Antibody
SKU:
CAB9278
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1509
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cytoplasm, Early Endosome, Endosome, Late Endosome, Membrane, Peripheral Membrane Protein.
Calculated MW:
92kDa
Observed MW:
92kDa
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
Purification Method
Affinity purification
Gene ID
55737
RRID
AB_2863704
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using VPS35 Rabbit mAb (CAB9278) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
