Based on the information provided in the URL, the VPS53 Antibody (PAC017431) is a polyclonal antibody that targets the VPS53 protein. VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, which plays a key role in the retrograde transport of proteins from endosomes to the trans-Golgi network. This antibody is specifically raised in rabbits and is highly reactive with human samples, making it suitable for use in various research applications.The VPS53 Antibody is validated for use in techniques such as Western blotting and immunofluorescence, enabling researchers to detect and analyze the expression and localization of VPS53 in different cell types.
This antibody is particularly useful for studies related to cell biology, protein trafficking, and organelle dynamics.Understanding the function and regulation of VPS53 is essential for unraveling its role in cellular processes and diseases. Dysregulation of the GARP complex, in which VPS53 is a critical component, has been implicated in various disorders, including neurodegenerative diseases, lysosomal storage disorders, and cancer. Research using the VPS53 Antibody can provide valuable insights into the mechanisms underlying these conditions and may lead to the development of novel therapeutic strategies targeting VPS53 and the GARP complex.
Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: A172 cell, Primary antibody: PACO17431(VPS53 Antibody) at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using PACO17431(VPS53 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
Background:
This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid, hydrolases such as CTSD.
Synonyms:
vacuolar protein sorting 53 homolog (S. cerevisiae)
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq, Jul 2008]
