The VPS54 Antibody (CAB13215) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By binding to the VPS54 protein, this antibody enables the detection and analysis of VPS54 in various cell types, making it ideal for investigations in cell biology and molecular biology research.VPS54 is a crucial component of the Golgi-associated retrograde protein (GARP) complex, which plays a vital role in the retrograde transport of proteins from endosomes to the trans-Golgi network.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
VPS54 Antibody
SKU:
CAB13215
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms
Purification Method
Affinity purification
Gene ID
51542
RRID
AB_2760068
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VPS54 Rabbit pAb (CAB13215) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
