The VTI1A Antibody (CAB5162) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets the VTI1A protein and is validated for use in Western blot applications. It offers high sensitivity and specificity, making it a reliable tool for studying VTI1A expression and localization in various cell types.VTI1A plays a crucial role in regulating vesicle fusion events within cells, contributing to processes such as protein transport and secretion. Dysregulation of VTI1A function has been implicated in various diseases, including neurodegenerative disorders and metabolic syndromes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
VTI1A Antibody
SKU:
CAB5162
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MMDS3, MVti1, VTI1RP2, Vti1-rp2, VTI1A
Positive Sample:
U-87MG, HT-1080, Mouse lung, Mouse kidney, Mouse brain, Rat lung
Cellular Localization:
Cytoplasmic Vesicle, Golgi Apparatus Membrane, Single-Pass Type Iv Membrane Protein.
Calculated MW:
25kDa
Observed MW:
30kDa
The protein encoded by this gene is a member of the family of soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptors (SNAREs) that function in intracellular trafficking. This family member is involved in vesicular transport between endosomes and the trans-Golgi network. It is a vesicle-associated SNARE (v-SNARE) that interacts with target membrane SNAREs (t-SNAREs). Polymorphisms in this gene have been associated with binocular function, and also with susceptibility to colorectal and lung cancers. A recurrent rearrangement has been found between this gene and the transcription factor 7-like 2 (TCF7L2) gene in colorectal cancers. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
143187
RRID
AB_2766041
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using VTI1A Rabbit pAb (CAB5162) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
