The WEE1 Monoclonal Antibody (CAB22275) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
This antibody is validated for use in IHC-P, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
WEE1 Monoclonal Antibody
SKU:
CAB22275
Size:
100μL, 20μL
Reactivity:
Mouse
Clone Number:
ARC57004
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
IHC-PELISA
Recommended Dilution:
IHC-P
1:1000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
WEE1A, WEE1hu, WEE1
Cellular Localization:
Cytoplasm, Nucleolus, Nucleoplasm, Nucleus.
Calculated MW:
72kDa
Observed MW:
Refer to figures
This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
Purification Method
Affinity purification
Gene ID
7465
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using WEE1 Rabbit mAb (CAB22275) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using WEE1 Rabbit mAb (CAB22275) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
