The WISP3 Antibody (CAB14812) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding to the WISP3 protein, this antibody enables the detection and analysis of WISP3 in various cell types, making it ideal for studies in bone biology and related fields.WISP3, also known as CCN6, is a member of the CCN (Cyr61, CTGF, Nov) family of matricellular proteins that play important roles in tissue development and repair. WISP3 specifically regulates processes related to bone formation and mineralization, making it a key target for research into skeletal disorders and conditions like osteoporosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
WISP3 Antibody
SKU:
CAB14812
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PPD, LIBC, PPAC, PPRD, WISP3, WISP-3
Positive Sample:
293T, HT-29, DU145, HepG2, Mouse brain, Rat brain
Cellular Localization:
Secreted.
Calculated MW:
39kDa
Observed MW:
41kDa
This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of this gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
8838
RRID
AB_2761690
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using WISP3 Rabbit pAb (CAB14812) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
