The WNK3 Polyclonal Antibody (PAC020908) is a valuable tool for researchers studying WNK3, a protein kinase involved in the regulation of ion transport and blood pressure. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting WNK3 in human samples, making it ideal for Western blot applications. By binding to the WNK3 protein, researchers can analyze its expression and activity in various cell types, providing insights into its role in cellular signaling pathways.
WNK3 is known for its involvement in the regulation of ion channels and transporters, particularly in the kidneys and nervous system. Dysregulation of WNK3 has been linked to hypertension, electrolyte imbalance, and neurological disorders, making it a significant target for pharmaceutical research and drug development. Understanding the function and regulation of WNK3 is essential for unraveling its role in disease pathogenesis and identifying potential therapeutic interventions in the future.
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using PACO20908(WNK3 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 6%SDS-PAGE,Lysate: 40 µg,Lane 1-2: MCF7 cells, 293T cells,Primary antibody: PACO20908(WNK3 Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 3 minutes.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20908(WNK3 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants.
Synonyms:
WNK lysine deficient protein kinase 3
UniProt Protein Function:
WNK3: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; Wnk family
Molecular Function:ATP binding; chloride channel inhibitor activity; protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: negative regulation of apoptosis; positive regulation of calcium ion transport; positive regulation of ion transmembrane transporter activity; protein amino acid autophosphorylation; protein amino acid phosphorylation
NCBI Summary:
This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
