The YTHDC1 Antibody (CAB7318) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reacts well with human samples, making it ideal for use in Western blot applications. By binding to the YTHDC1 protein, this antibody allows for the detection and analysis of YTHDC1 in various cell types, facilitating research in areas such as RNA processing, gene expression, and epigenetic regulation.YTHDC1 is an RNA-binding protein known for its role in mRNA splicing and processing, as well as its involvement in regulating alternative splicing events. Its functions in post-transcriptional gene regulation make it a crucial player in the control of gene expression and cellular processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
YTHDC1 Antibody
SKU:
CAB7318
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
MAAD SREE KDGE LNVL DDIL TEVP EQDD ELYN PESE QDKN EKKG SKRK SDRM ESTD TKRQ KPSV HSRQ LVSK PL
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
YT521, YT521-B, YTHDC1
Positive Sample:
HeLa, HCT116, 293F, RAW264.7, C6, Rat brain
Cellular Localization:
Nucleus.
Calculated MW:
85kDa
Observed MW:
110kDa
Enables N6-methyladenosine-containing RNA binding activity. Involved in mRNA export from nucleus; mRNA splice site selection; and regulation of gene expression. Located in nuclear speck and plasma membrane.
Purification Method
Affinity purification
Gene ID
91746
RRID
AB_2767858
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using YTHDC1 Rabbit pAb (CAB7318) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
