The Zinc-alpha2-glycoprotein (ZAG/AZGP1) Monoclonal Antibody (CAB11680) is a high-quality antibody developed for reliable detection and analysis of target proteins. Involved in cell adhesion and detection of chemical stimulus involved in sensory perception of bitter taste. Located in extracellular space.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ZAG, ZA2G, Zinc-α2-glycoprotein (ZAG/AZGP1)
Positive Sample:
Hep G2
Cellular Localization:
Secreted.
Calculated MW:
34 kDa
Observed MW:
35-45 kDa
Involved in cell adhesion and detection of chemical stimulus involved in sensory perception of bitter taste. Located in extracellular space.
Purification Method
Affinity purification
Gene ID
563
RRID
AB_2861627
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Hep G2 cells using Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (CAB11680) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit mAb (CAB11680) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
