The ZAK Antibody (CAB14513) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody exhibits high reactivity to human samples and is validated for use in Western blot applications. By specifically binding to the ZAK protein, researchers can effectively detect and analyze its expression in different cell types, making it an invaluable asset for studies in cell biology and molecular biology.ZAK, also known as sterile alpha motif and leucine zipper containing kinase, plays a crucial role in regulating cell proliferation, apoptosis, and stress response pathways.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ZAK Antibody
SKU:
CAB14513
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Mouse heart, Mouse brain, Mouse skeletal muscle, Rat heart, Rat skeletal muscle
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
35kDa/51kDa/91kDa
Observed MW:
105kDa
This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Purification Method
Affinity purification
Gene ID
51776
RRID
AB_2761388
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ZAK Rabbit pAb (CAB14513) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of L929 cells using ZAK Rabbit pAb (CAB14513) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
