The ZFYVE9 Antibody (CAB9041) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the ZFYVE9 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in cell biology and neuroscience.ZFYVE9 plays a crucial role in intracellular membrane trafficking and organelle dynamics, particularly in the regulation of endosomal compartments and neurite outgrowth.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ZFYVE9 Antibody
SKU:
CAB9041
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NSP, SARA, MADHIP, SMADIP, PPP1R173, ZFYVE9
Positive Sample:
LO2, HeLa
Cellular Localization:
Cytoplasm, Early Endosome Membrane.
Calculated MW:
156kDa
Observed MW:
156kDa
This gene encodes a double zinc finger motif-containing protein that participates in the transforming growth factor-beta (TGFB) signalling pathway. The encoded protein interacts directly with SMAD2 and SMAD3, and recruits SMAD2 to the TGFB receptor. There are multiple pseudogenes for this gene on chromosomes 2, 15, and X. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
9372
RRID
AB_2772962
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ZFYVE9 Rabbit pAb (CAB9041) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.