The SLC30A1 Antibody (CAB9954) is a high-quality antibody developed for reliable detection and analysis of target proteins. Zinc Transporter 1, also known as ZnT-1, plays a crucial role in regulating intracellular zinc levels and is essential for maintaining cellular function. This polyclonal antibody, raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.By binding specifically to Zinc Transporter 1 protein, this antibody enables researchers to detect and analyze ZnT-1 expression in various cell types. This makes it an ideal choice for investigations related to zinc homeostasis, metal ion transport, and cellular signaling pathways.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SLC30A1 Antibody
SKU:
CAB9954
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ZNT1, ZRC1, SLC30A1
Positive Sample:
Mouse kidney
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
55kDa
Observed MW:
55kDa
Predicted to enable calcium channel inhibitor activity and zinc ion transmembrane transporter activity. Predicted to be involved in several processes, including cellular divalent inorganic cation homeostasis; inorganic cation transmembrane transport; and negative regulation of transport. Predicted to act upstream of or within in utero embryonic development. Located in several cellular components, including Golgi apparatus; endoplasmic reticulum; and nuclear membrane.
Purification Method
Affinity purification
Gene ID
7779
RRID
AB_2772269
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SLC30A1 Rabbit pAb (CAB9954) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from mouse kidney, using SLC30A1 Rabbit pAb (CAB9954) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human stomach using SLC30A1 Rabbit pAb (CAB9954) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
